Nuclearity and BrdU Labeling of Rat Hepatocytes in Cytocentrifuge Preparations of Freshly Isolated Hepatocytes with Cumulative Labeling of Bromodeoxyuridine
スポンサーリンク
概要
- 論文の詳細を見る
Hepatocytes with 2 or more nuclei are seen at a certain percentage in the normal rat liver and show changes according to mitogenic stimuli including regeneration from injury. In the present study, analysis of nuclearity and cell kinetics of hepatocytes was conducted by combining cytocentrifuge preparations of isolated hepatocytes and bromodeoxyuridine (BrdU) cumulative labeling in 6-week-old female Sprague-Dawely rats at periods of 7, 14, and 28 days, respectively. In cytocentrifuge preparations, hepatocytes with intact cytoplasms were obtained, from which nuclearity was clearly and readily defined. The majority of hepatocytes were mononuclear or binuclear. The percentage of cells with 3 or more nuclei was very low. The percentages of each nuclearity class were similar throughout all labeling periods. The ratio of mononuclear to binuclear cells was approximately 6:4. The BrdU labeling index per total nuclei increased according to the labeling period. For the labeling index of each cell type, the number of BrdU positive mononuclear cells and binuclear cells with 2 positive nuclei (WP) increased according to the labeling period at a ratio of approximately 3:1. In the binuclear cells, WP cells and cells with 2 negative nuclei were dominant, whereas, cells with 1 positive and 1 negative nuclei were very rare. From these observations, the present method was thought to be well suited for analysis of nuclearity and cell kinetics in hepatocytes and may be useful for analysis of changes in the liver induced by various compounds.
- 日本毒性病理学会の論文
- 2004-03-30
著者
-
鈴木 雅実
Safety Assessment Dep. Fuji Gotemba Res. Laboratories Chugai Pharmaceutical Co. Ltd.
-
鈴木 雅実
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
-
Suzuki Masami
Safety Assessment Department, Fuji Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd.
-
Yamazaki Masaki
中外製薬富士御殿場研究所
-
Suzuki Masami
中外製薬富士御殿場研究所
-
Fujii Etsuko
中外製薬富士御殿場研究所
-
Fujii Etsuko
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
-
Fujii Etsuko
Safety Assessment Department Chugai Pharmaceutical
-
Fujii Etsuko
Toxicology Laboratory Chugai Pharmaceutical Co. Ltd.
-
Karasawa Yayoi
Safety Assessment Department, Chugai Pharmaceutical Co. Ltd.
-
Sugimoto Tetsuro
Safety Assessment Department, Chugai Pharmaceutical Co. Ltd.
-
杉本 哲朗
Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
-
Sugimoto Tetsuro
Toxicology Laboratory Chugai Pharmaceutical Co. Ltd.
-
Sakurai Takayuki
中外製薬安全性研究所
-
Sakurai Takayuki
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
-
MISAWA Yasuyuki
Safety Assessment Laboratory, Chugai Pharmaceutical Co., Ltd.
-
Yamazaki Masaki
Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
-
KUMANO Eiichi
Safety Assessment Department, Fuji Gotemba Research Labs., Chugai Pharmaceutical Co., Ltd.
-
MORI Toshio
Pharmacology and Pathology Research Center, Chugai Research Institute for Medical Science, Inc.
-
ITO Tsuneo
Pharmacology and Pathology Research Center, Chugai Research Institute for Medical Science, Inc.
-
Sakurai Takayuki
Safety Assessment Department Fuji Gotemba Research Labs. Chugai Pharmaceutical Co. Ltd.
-
Ito Tsuneo
Chugai Research Institute For Medical Science Inc.
-
Kumano Eiichi
Safety Assessment Department Fuji Gotemba Research Labs. Chugai Pharmaceutical Co. Ltd.
-
鈴木 雅実
Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
-
Karasawa Yayoi
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
-
Sugimoto Tetsuro
Safety Assessment Laboratory Chugai Pharmaceutical Co. Ltd.
-
Suzuki Masami
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
-
Ito Tsuneo
Safety Assessment Laboratory Chugai Pharmaceutical Co. Ltd.
-
Misawa Yasuyuki
Safety Assessment Department Fuji Gotemba Research Labs. Chugai Pharmaceutical Co. Ltd.
-
三沢 保幸
Toxicology Laboratory Chugai Pharmaceutical Co. Ltd.
-
FUJII Etsuko
PharmaLogicals Research Pte. Ltd.
-
三沢 保幸
Safety Assessment Laboratory Chugai Pharmaceutical Co. Ltd.
-
Misawa Yasuyuki
Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
-
Suzuki Masami
Safety Assessment Dep. Fuji Gotemba Res. Laboratories Chugai Pharmaceutical Co. Ltd.
-
Sugimoto Tetsuro
Safety Assessment Department Fuji Gotemba Research Labs. Chugai Pharmaceutical Co. Ltd.
-
Mori Toshio
Pharmacology And Pathology Research Center Chugai Research Institute For Medical Science Inc.
関連論文
- A method for sampling and tissue preparation of the parathyroid glands in miniature pigs for toxicity studies
- Cell proliferative activity in the kidney of young growing rat analyzed using flash and cumulative labeling with bromodeoxyuridine
- Differences in bone responses to recombinant human granulocyte colony-stimulating factor between mice and rats
- Effects of a Single Injection of Recombinant Human Granulocyte Colony-stimulating Factor on Bone in Rats
- Relation between Marrow Hematopoietic Activity and Development of Bone Changes Induced in Rats by Recombinant Human Granulocyte Colony-stimulating Factor
- Effects of erlotinib on lung injury induced by intratracheal administration of bleomycin (BLM) in rats
- Effects of erlotinib on lung injury induced by intratracheal administration of bleomycin (BLM) in rats
- Effects of T-2 Toxin on the Expression of Connexin 32 in the Mouse Liver
- Effects of T-2 Toxin Treatment on CCl_4-Induced Hepatic Necrosis in Mice
- Cell proliferative activity in the kidney of young growing rat analyzed using flash and cumulative labeling with bromodeoxyuridine
- Granulocyte Colony-Stimulating Factor Has No Adverse Effects on Atherosclerotic Lesions in High Cholesterol-Fed Miniature Swine(Pathology)
- Analysis by lectin histochemistry of kidney in Gottingen origin miniature pigs(Kidney, Urinary system, Proceedings of the 32nd Annual Meeting)
- Immunohistochemical detection of PTHrP and PTH/PTHrP receptor 1 on the odontoblastic reparative process after actinomycin D treatment in rats(Liver, Alimentary system, Proceedings of the 32nd Annual Meeting)
- A Decalcifying and Paraffin-embedding Method for Enzyme Histochemical and Immunohistochemical Studies of Bone(Proceedings of the 32nd Annual Meeting)
- COMBINATION OF FIXATION USING PLP FIXATIVE AND EMBEDDING IN PARAFFIN BY THE AMeX METHOD IS USEFUL FOR HISTOCHEMICAL STUDIES IN ASSESSMENT OF IMMUNOTOXICITY
- P12-11 The Combination of Fixation Using PLP Fixative and Embedding in Paraffin by the AMeX Method is Useful for Histopathological Studies in Assessment of Immunotoxicity.
- Teratologic Studies on Rat Perinates and Offspring from Dams Treated with Ethylnitrosourea (ENU)
- Histopathological Characteristics of Calcified Lesions of the Aortic Orifice (Ostium Aortae) in Dogs
- Tissue Distribution and Sequence of T-2 Toxin Induced-Apoptosis
- Changes in Location and Number of Tartrate-Resistant Acid Phosphatase (TRAP)-Positive Cells During the Development of Type II Collagen-Induced Arthritis in DBA/1J Mice
- Application of Flash and Cumulative Labeling with Bromodeoxyuridine for Analysis of Cell Kinetics of Bone Cells and Chondrocytes in Young Growing Rats
- Immunohistochemical Study on Type II Collagen-Induced Arthritis in DBA/1J Mice
- Immunohistochemical Study on Galactosamine-Induced Subacute Hepatitis in Rats of JCL: Wistar-TGN (ARGHGEN) 1Nts Strain (Mini rats)
- New measurement method of tissue factor (TF) activity in whole blood of cynomolgus monkeys (BLOOD SYSTEM) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- Application of coagulation and fibrinolytic tests for humans to cynomolgus monkeys.(GENERAL SESSION BY POSTER PRESENTATION)(BLOOD SYSTEM AND HEMATOLOGY)
- P-88 Collaborative work to evaluate toxicity on male reproductive organs by repeated dose studies in rats : Testicular toxicity of 2-or 4-week repeated or single-dose administration of a novel Platinum Complex(Proceedings of the 27th Annual Meeting)
- COLLABORATIVE WORK TO EVALUATE TOXICITY ON MALE REPRODUCTIVE ORGANS BY REPEATED DOSE STUDIES IN RATS : 11)TESTICULAR TOXICITY OF 2-OR 4-WEEK REPEATED OR SINGLE-DOSE ADMINISTRATION OF A NOVEL PLATINUM COMPLEX
- CONTRIBUTION OF INTESTINAL CALCIUM ABSORPTION TO 1,25-DIHYDROXYVITAMIN D_3-INDUCED CALCIUM ACTION IN THE TOTAL PARENTERAL NUTRITION RAT
- TOTAL PARENTERAL NUTRITION USING CONTINUOUS INTRAVENOUS INFUSION VIA THE POSTERIOR VENA CAVA IN RATS
- Changes of 1, 25 (OH)_2D_3-induced calcium action in the total parenteral nutrition feeding rat (examination of dose-dependent action)(General toxicity, Proceedings of the 32nd Annual Meeting)
- P7-40 Changes of 1,25(OH)_2D_3-induced calcium action in the rat total parenteral nutrition model(GENERAL TOXICITY)(GENERAL SESSION BY POSTER PRESENTATION)(Proceedings of the 31st Annual Meeting)
- O12-07 Time Course Changes of Sorbitol Dehydrogenase. Glutamate Dehydrogenase, 5'-Nucleotidase and Total Serum Bile Acids During Acute Liver Injury in Rat
- 3B-08 Changes in Serum C-Reactive Protein (CRP) in Experimental Gastropathy
- Nuclearity and BrdU Labeling of Rat Hepatocytes in Cytocentrifuge Preparations of Freshly Isolated Hepatocytes with Cumulative Labeling of Bromodeoxyuridine
- PROLONGATION OF PT AND APTT UNDER EXCESSIVE ANTICOAGULANT IN PLASMA FROM RATS AND DOGS
- Development of a Novel Model for Streptozotocin-induced Renal Cell Tumors and Chronic Diabetes in Gottingen Minipigs
- Lobular Difference in Fibrotic Changes in Rat Cirrhosis Model Induced by Carbon Tetrachloride
- PLP-AMeX Method, Fixation Using PLP Fixative and Embedding in Paraffin by the AMeX Method, is Useful not only for Histochemistry but also In Situ Hybridization
- Recovery of blood and urine Ca and IP level after low phosphorus state.(GENERAL SESSION BY POSTER PRESENTATION)(LABORATORY ANIMAL TECHNIQUES)
- Immunohistochemical Identification of T- and B-Lymphocytes in Formalin-Fixed, Paraffin-Embedded Rat Sections
- Collaborative work on evaluation of ovarian toxicity : 10) Two- or four-week repeated dose studies and fertility study of di-(2-ethylhexyl) phthalate (DEHP) in female rats
- Immunohistochemical Detection of PTHrP and PTH/PTHrP Receptor 1 on the Odontoblastic Reparative Process after Actinomycin D Treatment in Rats
- HEMODYNAMIC AND ELECTROPHYSIOLOGICAL EFFECTS OF MITEMCINAL (GM-611), A NOVEL PROKINETIC AGENT DERIVED FROM ERYTHROMYCIN IN A HALOTHANE-ANESTHETIZED CANINE MODEL
- PRECLINICAL ELECTROPHYSIOLOGY ASSAYS OF MITEMCINAL (GM-611), A NOVEL PROKINETIC AGENT DERIVED FROM ERYTHROMYCIN
- Interferon-α enhances CD317 expression and the antitumor activity of anti-CD317 monoclonal antibody in renal cell carcinoma xenograft models
- HISTOPATHOLOGICAL STUDY OF THE EFFECTS OF A SINGLE INTRATRACHEAL INSTILLATION OF SURFACE ACTIVE AGENTS ON LUNG IN RATS
- THE EFFECT OF FOOD DYE AND OTHER ENVIRONMENTAL SUBSTANCES ON THE HOST DEFENSE REACTION IN MICE IN RELATION TO VIRUS INFECTION
- EFFECTS OF LYSINE-INDUCED ACUTE RENAL FAILURE IN DOGS
- Application of the continuous intravenous infusion to total parenteral nutrition in rats.(GENERAL SESSION BY POSTER PRESENTATION)(LABORATORY ANIMAL TECHNIQUES)
- P11-07 Changes of non-protein nitrogen and blood urea nitrogen as renal failure parameters by the amino acid toad
- The basic examination of urinary protein fractions using SDS-PAGE in cynomolgus monkeys.(GENERAL SESSION BY POSTER PRESENTATION)(KIDNEYS AND URINARY EXCRETION SYSTEM)
- Induction and Inhibition of Cytochrome P450 and Drug-Metabolizing Enzymes by Climbazole
- CLIMBAZOLE IS A NEW POTENT INDUCER OF RAT HEPATIC CYTOCHROME P450
- SEX-RELATED EFFECT OF HEMIN ON CYTOCHROME P450 AND DRUG-METABOLIZING ENZYMES IN RAT LIVER
- "A-6 Immunohistochemical study on the component cells and extracellular matrix in galactosamine- induced acute hepatitis in rats.
- Changes in serum α1-acid glycoprotein and interleukin-8 in experimental lung injury in rats
- Segmentation of the Pathophysiological Stages of Diabetic Changes in the db/db Mouse
- The Initial Lesions Caused by Sodium Chloride in Canine Gastric Mucosa
- Establishment and characterization of in vivo human tumor models in the NOD/SCID/γ_c^ mouse
- "P-40 The Age-related Difference in Bone Changes in Rats induced by Recombinant Granulocyte Colony-Stimulating Factor
- Enhancement of paraquat toxicity by glutathione depletion in mice in vivo and in vitro
- Effect of Glutathione Depletion on Metallothionein Synthesis Induced by Paraquat in Mice
- Glutathione Depletion Enhances Induction of Metallothionein Synthesis by Paraquat (Proceedings of the 19th Symposium on Toxicology and Environmental Health)
- DISTRIBUTION OF ^C-A-145-AN ANTITUMOR AMINO ACID DERIVATIVE-IN MICE (The Fourth Meeting for the Study of Toxic Effect)
- 21D-01-5 Selective increase of cyclooxygenase-2 expression in the remnant kidney in the rat.
- 21D-01-1 In vitro nephrotoxicity assay system and its application to high throughput screening
- O-27 The circadian rhythm of blood pressure disappears in rats with chronic renal failure(Proceedings of the 27th Annual Meeting)
- The Combination of Fixation Using PLP Fixative and Embedding in Paraffin by the AMeX Method is Useful for Immunohistochemical and Enzyme Histochemical Studies of the Lung
- Endometriosis in a Rhesus Monkey
- Effects of Granulocyte Colony-Stimulating Factor on the Development of Inflammation in Bleomycin-Induced Lung Injury
- Segmentation of the Pathophysiological Stages of Diabetic Changes in the db/db Mouse
- Emphysematous Cystitis in a Chemically-Induced Diabetic Dog
- W2-3 USE OF PRIMARY CULTURED HEPATOCYTES FOR TOXICOLOGY