Immunohistochemical Identification of T- and B-Lymphocytes in Formalin-Fixed, Paraffin-Embedded Rat Sections
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概要
- 論文の詳細を見る
We investigated the possibility of immunohistochemically identifying T- and B-lymphocytes in formalin-fixed, paraffin-embedded rat tissues as a basis for future pathological study. Spleens were removed from 8-week-old male Wister rats, and were fixed in 20% neutral-buffered formalin for 7, 30 or 90 days. After fixation, the specimens were embedded in ordinary paraffin by routine method. Paraffin sections were prepared and stained immunohistochemically using anti-CD3 (clone: G4.18) and -CD45RA (clone: OX-33) antibodies. Anti-CD3 and -CD45RA antibodies failed to stain without microwave heating pretreatment. The immunoreactivity of CD3 and CD45RA were both increased by microwave heating pretreatment. Consistent and specific immunostaining of CD3 was obtained throughout all formalin-fixation periods after 40 min or more of microwave heating pretreatment. CD45RA was clearly detected in the 7-day fixed tissues after 30 or 40 min of pretreatment. In the 30- and 90-day fixed tissues, identification of CD45RA was difficult at all lengths of microwave heating pretreatment. These findings suggest that CD3 and CD45RA are detectable in routine formalin-fixed, paraffin-embedded rat tissues although the immunoreactivity is influenced by formalin-fixation period, and that the present procedure, combining appropriate antibodies and pretreatment of microwave heating, is useful for retrospective analyses of rat lymphoid subtypes.
- 日本毒性病理学会の論文
- 2002-09-25
著者
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鈴木 雅実
Safety Assessment Dep. Fuji Gotemba Res. Laboratories Chugai Pharmaceutical Co. Ltd.
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鈴木 雅実
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
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Yamazaki Masaki
中外製薬富士御殿場研究所
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Suzuki Masami
中外製薬富士御殿場研究所
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Fujii Etsuko
中外製薬富士御殿場研究所
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Fujii Etsuko
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
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Fujii Etsuko
Toxicology Laboratory Chugai Pharmaceutical Co. Ltd.
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SUZUKI Masami
Toxicology Laboratory, Chugai Pharmaceutical Co., Ltd.
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MISAWA Yasuyuki
Toxicology Laboratory, Chugai Pharmaceutical Co., Ltd.
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SUGIMOTO Tetsuro
Toxicology Laboratory, Chugai Pharmaceutical Co., Ltd.
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杉本 哲朗
Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
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Sugimoto Tetsuro
Toxicology Laboratory Chugai Pharmaceutical Co. Ltd.
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Sugimoto Tetsuro
Toxicol.res.labs. Chugai Pharmaceu.co. Ltd.
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Sakurai Takayuki
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
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Yamazaki Masaki
Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
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Sakurai Takayuki
Safety Assessment Department Fuji Gotemba Research Labs. Chugai Pharmaceutical Co. Ltd.
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鈴木 雅実
Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
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TERUYA Shino
Department of Toxicology, Fuji Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd.
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TERUYA Shino
Toxicology Laboratory, Chugai Pharmaceutical Co., Ltd.
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Teruya Shino
Department Of Toxicology Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
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Misawa Yasuyuki
Toxicology Lab. Chugai Pharm. Co. Ltd.
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Sugimoto Tetsuro
Safety Assessment Laboratory Chugai Pharmaceutical Co. Ltd.
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Suzuki Masami
Toxicology Laboratory Chugai Pharmaceutical Co. Ltd.
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Suzuki Masami
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
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三沢 保幸
Toxicology Laboratory Chugai Pharmaceutical Co. Ltd.
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FUJII Etsuko
PharmaLogicals Research Pte. Ltd.
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三沢 保幸
Safety Assessment Laboratory Chugai Pharmaceutical Co. Ltd.
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Misawa Yasuyuki
Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
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Suzuki Masami
Toxicol. Res. Labs. Chugai Pharmaceu. Cp. Ltd.
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