動物種の如何を問わずE型肝炎ウイルス抗体を検出し得る簡便ELISA法

概要

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A partial ORF2 protein of hepatitis E virus (HEV) was expressed in E. coli, purified, and used as solid-phase ligand as well as tracer-ligand labeled with peroxidase, in an antigen-antibody-antigen Sandwich ELISA system, to detect antibodies to HEV. This new system showed higher sensitivity of detecting anti-HEV than conventional ELISA system which needs specific antibodies against IgG, IgM, or IgA of concerned animal species as tracer-ligands. Since our new ELISA system is universally applicable to any animal species without needing specific antibodies, it will be useful in further elucidating the animal reservoirs of HEV.
社団法人　日本肝臓学会の論文
2007-07-25