Alkalinization-Induced K^+ Current of the Mouse Megakaryocyte

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概要

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• We have recently found that mouse megakaryocytes responded to extracellular alkalinization to pH>8.0, generating a K+ current under voltage-clamped conditions with the whole cell recording mode of the patch-clamp technique. The purpose of this study was to physiologically and pharmacologically characterize the alkaline-dependent K+ conductance of the megakaryocyte membrane. The alkalinization-induced K+ current (IALK) did not seem to be Ca2+-dependent since IALK was allowed to be generated under intracellularly Ca2+-buffered conditions with 10 mM EGTA, which completely prevented the generation of caffeine-induced Ca2+-activated currents of mouse megakaryocytes; and no [Ca2+]i elevation was evoked by the alkalinization protocol in contrast to a significant increase in [Ca2+]i in response to caffeine when [Ca2+]i was measured with a fura 2 ratiometry. IALK was strongly suppressed with tetraethylammonium (TEA), 4-aminopyridine (4-AP) and streptomycin (SM), but was completely resistant to quinidine (QND). The values of IC50 for the suppression of IALK with TEA, 4-AP and SM were 5.6, 0.47 and 1.5 mM, respectively. Voltage-gated K+ currents (IK) of the same megakaryocyte preparation were weakly suppressed with TEA and 4-AP, while they were significantly suppressed with either SM or QND. These results suggest that mouse megakaryocytes possess K+ conductance that was activated by extracellular alkalinization and that probably differs from conventional K+ conductance in its pharmacological properties.

• 社団法人 日本薬理学会の論文
• 1999-03-01

著者

• Tomita Kimio

  Department Of Nephrology Kumamoto University Graduate School Of Medical Science

• MURAKAMI Makoto

  Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University

• Tomita Kimio

  Third Department Of Internal Medicine Kumamoto University School Of Medicine

• Tomita Kimio

  Departments Of Internal Medicine Kumamoto University School Of Medicine

• 西 勝英

  熊本大学医学薬学研究部生体機能薬理学教室

• Tomita Kimio

  Department Of Nephrology Graduate School Of Medical Sciences Kumamoto University

• 徳冨 芳子

  Graduate School Of Medical Sciences Kumamoto University

• 徳冨 直史

  Graduate School Of Medical Sciences Kumamoto University

• TOKUTOMI Naofumi

  Pharmacology, Kumamoto University School of Medicine

• TOKUTOMI Yoshiko

  Pharmacology, Kumamoto University School of Medicine

• NISHI Katsuhide

  Pharmacology, Kumamoto University School of Medicine

• Murakami Makoto

  Department Of Agricultural Engineering Faculty Of Agriculture Hokkaido University

• Murakami Makoto

  Departments Of Internal Medicine Kumamoto University School Of Medicine

• Nishi Katsuhide

  Department Of Biopharmaceutics Graduate School Of Pharmaceutical Sciences Kumamoto University

• Tomita Kimio

  Department Of Nephrology Faculty Of Life Science Kumamoto University

• Tomita Kimio

  Department of Medicine, Tokyo Medical and Dental University

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