Effects of Sasanquasaponin on Ischemia and Reperfusion Injury in Mouse Hearts
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概要
- 論文の詳細を見る
We investigated effects of sasanquasaponin (SQS), a traditional Chinese herb’s effective component, on ischemia and reperfusion injury in mouse hearts and the possible role of intracellular Cl− homeostasis on SQS’s protective effects during ischemia and reperfusion. An in vivo experimental ischemia model was made in mice (weight 27 – 45 g) using ligation of left anterior descending coronary artery, and in vitro models were made in perfused hearts by stopping flow or in isolated ventricular myocytes by hypoxia. The in vivo results showed that SQS inhibited cardiac arrhythmias during ischemia and reperfusion. Incidence of arrhythmias during ischemia and reperfusion, including ventricular premature beats and ventricular fibrillation, was significantly decreased in the SQS-pretreated group (P<0.05). Results in perfused hearts showed that SQS suppressed the arrhythmias, prevented against ischemia-induced decrease in contract force and promoted the force recovery from reperfusion. Furthermore, intracellular Cl− concentrations ([Cl−]i) were measured using a MQAE fluorescence method in isolated ventricular myocytes in vitro. SQS slightly decreased [Cl−]i in non-hypoxic myocytes and delayed the hypoxia/reoxygenation-induced increase in [Cl−]i during ischemia and reperfusion (P<0.05). Our results showed that SQS protected against ischemia/reperfusion-induced cardiac injury in mouse hearts and that modulation of intracellular Cl− homeostasis by SQS would play a role in its anti-arrhythmia effects during ischemia and reperfusion.
- 社団法人 日本薬理学会の論文
- 2004-03-01
著者
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Lai Zhong-fang
Department Of Cell And Biological Pharmacology Kumamoto University
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HE MING
Department of Chemical Engineering, Nanjing University of Technology
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Chen Yu-zhen
Department Of Immunogenetics Graduate School Of Medical Sciences Kumamoto University
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NISHI KATSUHIDE
Department of Pharmacology, Kumamoto University Medical School, Kumamoto, Japan
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He Ming
Department Of Biochemistry And Molecular Biology Hebei Medical University
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He Ming
Department Of Pharmacology Jiangxi Medical College
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SHAO Zhanqiang
Department of Cell and Biological Pharmacology, Kumamoto University
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HUANG Qiren
Department of Pharmacology, Jiangxi Medical College
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Nishi Katsuhide
Department Of Cell And Biological Pharmacology Kumamoto University
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Huang Qiren
Department Of Pharmacology Jiangxi Medical College
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Shao Zhanqiang
Department Of Cell And Biological Pharmacology Kumamoto University
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Nishi Katsuhide
Department Of Biopharmaceutics Graduate School Of Pharmaceutical Sciences Kumamoto University
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