The Effect of Sex Hormones on Peroxisome Proliferator-Activated Receptor Gamma Expression and Activity in Mature Adipocytes
スポンサーリンク
概要
- 論文の詳細を見る
Peroxisome proliferator-activated receptor (PPAR) γ plays a major role in the regulation of lipid and carbohydrate metabolism. Pioglitazone is a PPARγ agonist that is widely used for the treatment of type 2 diabetes mellitus. However, female patients have been reported to experience stronger efficacy and adverse effects than male patients. This study evaluated the effects of sex hormones on PPARγ expression and activity in adipocytes. Mouse 3T3-L1 preadipocytes were used after being grown into matured adipocytes. The sex hormones 17β-estradiol (E2), testosterone (T), or 5α-androstan-17β-ol-3-one (dihydrotestosterone; DHT) were added to the matured adipocytes and the cells were then maintained for short (24–72 h) or long (1- or 2-weeks) periods. E2 significantly upregulated PPARγ protein expression in a concentration-dependent manner after extended exposure, whereas T and DHT did not have such an effect. When cells were co-treated with pioglitazone and E2, PPARγ protein expression significantly increased in an E2-dependent manner, whereas this expression seemed to be reduced by pioglitazone mono-treatment and co-treatment with DHT at higher concentrations. The secretion levels of adiponectin protein, a major indicator of PPARγ activity, were significantly decreased by DHT, but were not affected by E2. Finally a luciferase assay was performed using a PPAR response element-Luk reporter gene. Transcriptional activity was not changed by any of single sex hormone treatment, but was significantly downregulated by co-treatment with pioglitazone and DHT. Taken together, our results suggest that sex hormones may influence PPARγ expression and function, which may explain the observed sex-specific different effect of pioglitazone.
著者
-
Kimura Yuki
Department Of Applied Physics University Of Miyazaki
-
Ishikawa Momoko
Department Of Gastroenterology Institute Of Health Biosciences University Of Tokushima Graduate Scho
-
Sato Hiromi
Department of Geriatric Pharmacology and Therapeutics, Graduate School of Pharmaceutical Sciences, Chiba University
-
Sato Hiromi
Department of Applied Chemistry, Graduate School of Engineering, Tohoku University, 6-6-11-1302 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan
-
UENO Koichi
Department of Biochemical Pharmacology and Biotoxicology, Faculty of Pharmaceutical Sciences, Chiba University
-
Funaki Asami
Department of Geriatric Pharmacology and Therapeutics, Graduate School of Pharmaceutical Sciences, Chiba University
-
Sugai Hana
Department of Geriatric Pharmacology and Therapeutics, Graduate School of Pharmaceutical Sciences, Chiba University
-
Kurosaki Hiroshi
Department of Geriatric Pharmacology and Therapeutics, Graduate School of Pharmaceutical Sciences, Chiba University
関連論文
- Expression of histamine H4 receptor in human epidermal tissues and attenuation of experimental pruritus using H4 receptor antagonist
- The structure-activity relationship between oxycoumarin derivatives showing inhibitory effects on iNOS in mouse macrophage RAW264.7 cells
- Toxicokintic and toxicodynamic analysis of clofibrate based on free drug concentrations in nagase analbuminemia rats (NAR)
- Enhanced Effect of Connexin 43 on Cisplatin-Induced Cytotoxicity in Mesothelioma Cells
- Energy-Scale Calibration of the Suzaku X-Ray Imaging Spectrometer Using the Checker Flag Charge-Injection Technique in Orbit
- A Patient with Early Syphilis Complicated by Fatty Liver Who Showed an Alleviation of Hepatopathy Accompanied by Jaundice after Receiving Anti-syphilitic Therapy
- PE-415 Induction of Diacylglycerol O-acyltransferase 1 Gene Induces Tumor Necrosis Factor-α Gene Expression in 3T3-L1 Adipocytes(Diabetes/Obesity/Metabolic syndrome-06, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Surgical Treatment of Renal Cell Carcinoma with Tumor Thrombi in the Inferior Vena Cava
- A COMPARATIVE STUDY OF HISTAMINE ACTIVITIES ON DIFFERENTIATION OF OSTEOBLASTS AND OSTEOCLASTS
- Establishment and Pathophysiological Characterization of Type 2 Diabetic Mouse Model Produced by Streptozotocin and Nicotinamide(Highlighted paper selected by Editor-in-chief,Pharmacology)
- Comparative study of histamine H4 receptor expression in human dermal fibroblasts
- Expression of Histamine H4 Receptor in Synovial Cells from Rheumatoid Arthritic Patients(Biopharmacy)
- Histamine H4 Receptor Expression in Human Synovial Cells Obtained from Patients Suffering from Rheumatoid Arthritis(Biopharmacy)
- Successful treatment of pulmonary metastases associated with advanced hepatocellular carcinoma by systemic 5-fluorouracil combined with interferon-α in a hemodialysis patient
- Cytotoxic Effect of the Her-2/Her-1 Inhibitor PKI-166 on Renal Cancer Cells Expressing the Connexin 32 Gene
- THE EFFECTS OF ASPIRIN AND ITS METABOLITE, SALICYLIC ACID, ON CULTURED RAT EMBRYO
- ACETYLSALICYLIC ACID INDUCED FETALTOXICITY AND DRUG METABOLISM
- Decrease in protein binding and its effect on toxicokinetics (TK)/toxicodynamics (TD) of diclofenac and propranolol in pregnant rats
- Atorvastatin decreases serum levels of advanced glycation endproducts (AGEs) in nonalcoholic steatohepatitis (NASH) patients with dyslipidemia : clinical usefulness of AGEs as a biomarker for the attenuation of NASH
- Phase Transition Temperature of γ-Fe_2O_3 Ultrafine Particle(Cross-disciplinary Physics and Related Areas of Science and Technology)
- Pentagonal Configurartion in GaP Ultrafine Particle
- Induction of Cytochrome P450 1A1 in Mice by Repeated Oral Administration of Propranolol
- Reduction of Paraquat-induced Oxidative Stress in Rats by Dietary Soy Peptide(Food & Nutrition Science)
- 軽度認知機能障害スクリーニングに対するコンピュータ検査システムの評価
- Analysis of Correlation between Prescription Drugs for Climacteric Disorders and CA Repeat Polymorphism of Estrogen Receptor β Gene
- 2-Aryl-1,4-naphthoquinone-1-oxime Methyl Ethers: Their Cytotoxic Activity
- Neonatal Orbital Teratoma with Intracranial Extension : -Case Report-
- Effect of Nonspecific Binding to Microsomes and Metabolic Elimination of Buprenorphine on the Inhibition of Cytochrome P4502D6(Biochemistry/Molecular Biology)
- Inhibitory effect of the extracts from Thai medicinal plants on iNOS expression in mouse macrophage RAW 264.7
- Inhibitory effect of oxycoumarins isolated from the Thai medicinal plant Clausena guillauminii on the inflammation mediators, iNOS, TNF-α, and COX-2 expression in mouse macrophage RAW 264.7
- Sex Differences in PPARγ Expressions in Rat Adipose Tissues(Biopharmacy)
- Morphological characteristics of Nigorobuna Carassius auratus grandoculis called Io in Lake Nishino
- Reducing Effect of Feeding Powdered Nacre of Pinctada maxima on the Visceral Fat of Rats
- INFLUENCE OF MATERNAL DRUG METABOLISM ON THE FETAL TOXICITY INDUCED BY ACETYLSALICYLIC ACID
- A case of a 2-year-old boy with tuberous sclerosis complicated with descending aortic aneurysm
- Development of cPHARM-2E, a Computerized Medical Incident Analysis System
- Zoledronic acid delays disease progression of bone metastases from hepatocellular carcinoma
- A-55. Arteriovenous Malformation of Vertebro-Basilar Tree Special Emphasis on its Classification and Operative Indcation
- Alteration of interstellar organic materials in meteorites parent bodies : a novel route for diamond formation
- Formation of polycyclic aromatic hydrocarbon grains using anthracene and their stability under UV irradiation
- METHYLENETETRAHYDROFOLATE REDUCTASE AND REDUCED FOLATE CARRIER-1 GENOTYPES AND METHOTREXATE SERUM CONCENTRATIONS IN PATIENTS WITH RHEUMATOID ARTHRITIS
- Effects of N-acetyltransferase 2 (NAT2), CYP2E1 and Glutathione-S-transferase (GST) genotypes on the serum concentrations of isoniazid and metabolites in tuberculosis patients
- Reactions of N(2^2D)with Alkane Hydrocarbons to Produce NH(X^3Σ^-)
- Evaluation of Carcinogenic/Co-carcinogenic Activity of Chikusaku-eki, a Bamboo Charcoal By-product Used as a Folk Remedy, in BALB/c3T3 Cells
- A novel animal model of pruritus induced by successive application of glucocorticoid to mouse skin
- Development of a Multi-Scale Electromigration Simulator Based on a Combination of Ultra Accelerated Quantum Chemical Molecular Dynamics and Kinetic Monte Carlo Methods Application to Cu Interconnects Lifetime Simulation
- Acetaminophen enhances pruritus in a mouse model of contact dermatitis induced by suboptimal concentration of hapten
- The Histamine H_2-Receptor Antagonist, Cimetidine, Inhibits the Articular Osteopenia in Rats With Adjuvant-Induced Arthritis by Suppressing the Osteoclast Differentiation Induced by Histamine
- Production of Chimeric Loach by Cell Transplantation from Genetically Pigmented to Orange Embryos(Developmental Biology)
- Remarkable Expansion of the Poly(acrylic acid) Chain by Acid-Base Complexation with Low Molecular Weight Amines
- New 2-Aryl-1,4-naphthoquinone-1-oxime Methyl Ether Compound Induces Microtubule Depolymerization and Subsequent Apoptosis
- Multi-angle observation scheme for bubbles and droplets
- Sex-related differences in pharmacokinetics and pharmacodynamics of anti-hypertensive drugs
- The spatial contrast suppression is polarity selective(Summary of Awarded Presentation at the 30th Annual Meeting)
- Structure and Thickness of Natural Oxide Layer on Ultrafine Particle
- Growth of Diamond from Si-Containing Amorphous Carbon Film Using Synchrotron Radiation
- Structural Control of Silicon Oxide Particles by Oxygen Partial Pressure in RF Plasma
- Repeated application of glucocorticoids exacerbate pruritus via inhibition of prostaglandin D_2 production of mast cells in a murine model of allergic contact dermatitis
- The Effect of Sex Hormones on Peroxisome Proliferator-Activated Receptor Gamma Expression and Activity in Mature Adipocytes
- Plasma glutathione S-transferase in carbon tetrachrolide treated rats and its association to hepatic cytosolic isozymes.
- Potentiality of application of triploidy to the culture of Ayu, Plecoglossus altivelis temminck et Schlegel.
- Chromosome Studies of Two Species of the Genus Coreoperca (Pisces: Perciformes), with Reference to the Karyotypic Differentiation and Evolution
- Hyperinsulinemia Leads the Elevation of Plasma Aldosterone Concentration Independently with Obesity, Dyslipidemia, and Insulin Resistance in Patients with Type 2 Diabetes Mellitus
- Interferometric Observation of Temperature Distributions in the Smoke Experiment