An estimation of the molecular weight and location of maxillary cancer associated antigen.

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It is well known that the early diagnosis of maxillary cancer (MC) is very difficult on the basis of its clinical appearance and symptoms, since nasal obstraction, bloody discharge, toothache and loosening of the teeth are common in the early stage.A new technique was devised for the isolation and charactrization of maxillary cancer associated antigens (MCAA), since elucidation of MCAA has been one of the major achievements of tumor immunology.Frozen sections of MC 10μm thick were applied directly to PAG-plates for isoelectric focusing. Saline extract of tumor tissues and sera were treated in the same manner.Specific components at around pH 6.1 were determined on PAG-plates and collected by chromatofocusing. Anti-MCAA rabbit serum was obtained by immunization with the components around pH 6.1 and anti-MCAA IgG serum was purified by protein A sepharose CL-4B.The purpose of this study was to demonstrate the location of MCAA in tumor sections and determine its approximate molecular weight. Tissues of 10 out of 11 (91%) cases of MC were stained positively by indirect immunofluorescence and immunoperoxidase techniques. This definitely positive staining was demonstrated on the cell membranes of MC, while in 8 control cases (normal maxillary sinus membrane, paratine tonsil, parotid gland and membranes from patients with chronic paranasal sinusitis) the staining was definitively negative.The molecular weight of MCAA was investigated by SDS-PAG and Immune-Blotting. The former showed two components (170k-225k), and the latter detected two components (120k-136k) and one minor component of 76k.
耳鼻咽喉科臨床学会の論文

An estimation of the molecular weight and location of maxillary cancer associated antigen.

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