Aeromonas salmonicidaの菌体外産生物質に対するエゾイワナの液性免疫応答〔英文〕

概要

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White-spotted char Salvelinus leucomaenis were immunized intramuscularly with Aeromonas salmonicida extracellular products (ECP). By immunization two or three times at four week intervals, the serum agglutinating antibody titers at twelve weeks after the first immunization were raised ranging from 1:128 to 1:512 or from 1:512 to 1:4, 096 respectively. Disc electrophoretic analysis did not show any difference in the protein composition between im-munized and non-immunized fish sera. By immunodiffusion analysis, char anti-ECP sera formed a single precipitating line against ECP and salmolysin. The component protein of the char antisera, which reacted to ECP, also formed a single precipitating line against ECP in β to γ-globulin region by immunoelectrophoresis. The antiserum was separated into three frac-tions by gel filtration on Sepharose 6B, and antibody activity was found in early eluate of the second peak with Kav of 0.4 to 0.6. The results indicate that salmolysin, which is included in ECP, possesses high immunogenicity to white-spotted char.
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