アユにおける溶菌性物質の分布ならびに性状

概要

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The bacteriolytic activity was examined on the homogenates of some organs of Ayu Pleco-glosses altivelis: the mucus on the body surface, kidney, intestine, mucus on gill surface and serum. The enzymatic properties of the bacteriolytic activity of the skin mucus and kidney were also in-vestigated. The activity was determined by the turbidimetric method using acetone-ether-dried sells of Micrococcus lysodeikticus as the substrate. The skin mucus showed the highest bacteriolytic activity, followed in order by the kidney, intestine and gill mucus, but the serum didn't lyse the cells. The maximal activity of the bacterio-lysis by the skin mucus appeared in 0.0075M phosphate buffer of pH 6.5 at 35°C. And the kidney showed the maximal activity in 0.01M phosphate buffer of pH 7.0 at 35°C. The bacterio-lytic activity of both the skin mucus and the kidney heated at 100°C for 10 min in the acidic buffer (pH 3.5) were relatively stable but those in neutral (pH 7.0) or the alkaline (pH 9.0) buffer were liable to be inactivated, as shown by the lysozyme from the hen egg-white.
日本水産學會の論文