ヒジキのクロロフィル-タンパク質複合体の分離と色素組成

概要

論文の詳細を見る
Six pigmented zones were separated from samples of chloroplast of brown algae Hizikia fusiformis by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The resolved zones in order of increasing mobility were: I (green), II (green), III (yellow green) and IV (brown orange) (these four were chlorophyll-protein complexes (CPC)) as well as zone V (green) and zone VI (orange), which were free pigments in the non-protein part. The slowest migrating zone (I) contained chlorophyll (Chl) a and β-carotene, and had similar properties to the Chi a-protein complex (CPl) isolated from other brown algae in the absorption and fluorescence spectra. Zone II contained Chl a, Chl c and fucoxanthin, while III contained Chl a, Chl c, fucoxanthin and very little other xanthophyll. The fastest migrating (IV: brown orange zone) contained Chl a, Chl c and fucoxanthin, and showed efficient energy transfer from Chl c and fucoxanthin to Chl a. The brown orange complex of IV might be the major Chl a-Chl c-fucoxanthin protein complex in H. fusiformis chloroplast and play the role of the light harvesting complex.