コイ肝膵臓におけるphospholipase A2 のワックスエステル合成に与える影響

概要

論文の詳細を見る
The hydrolysis of L-1-palmitoyl-2- [1-14C] arachidonyl-3-sn-glycerophosphatidylcholine (14C-PC) was studied with carp hepatopancreas preparation. Phospholipase A2 of 10, 000×g supernatant showed the highest activity around 35 to 45°C at pH 5.0. The enzyme was activated by the addition of Ca2+ (10mM), sodium deoxycholate (5.0mM) and Triton X-100 (10mM) to the reaction mixture, whereas ethylenediaminetetraacetic acid (EDTA, 0.5mM) did not stimulate the activity. Some parts of labelled acid released from 14C-PC were incorporated into triglycerides, partial glycerides, and wax esters. The incorporation of radioactivity into wax esters was promoted by the addition of oleyl alcohol. Both the hydrolyzing activity of 14C-PC and the synthesizing activity of wax ester from 14C-PC were distributed in 700×g pellet, lipid cap, 8, 000×g pellet (mitochondrial fraction), 105, 000×g pellet (microsomal fraction), and 105, 000×g pellet supernatant. Moreover, the role of phospholipase A2 on wax ester synthesis in carp hepatopancreas preparation was discussed.
公益社団法人日本水産学会の論文