Fatty Acid Metabolism in Blue Crab, Portunus trituberculatus, with Special Reference to De Novo Synthesis and Conversion

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Japanese blue crab, Portunus trituberculatus, contains 1.6% of lipids as whole body with particularly high content (12.3%) in hepatopancreas and approximately 1% in muscle in the wet weight basis. The lipids of various tissues consist mainly of triglycerides, phospholipids, free fatty acids and diacyl glyceryl ethers. In order to determine the fatty acid metabolism in crab, [1-14C] acetate (200μCi of sodium salt dissolved in physiological saline) was given intraperitoneally and the crab was sacrificed by freezing at 5h after injection. Following the extraction of lipids, the saturated, monounsaturated, and polyunsaturated fatty acids were concentrated by low temperature crystallization from acetone and separated by argentation thin-layer and reversed-phase column chromatographies. The unsaturated acids were degraded by oxidative ozonolysis. Palmitic acid, stearic acid which was derived from oleic acid by hydrogenation, and arachidic acid derived from icosapentaenoic acid were respectively degraded stepwise from carboxyl carbon, and the distribution of percentage radioactivities was determined in each molecule. The results indicate that the crab de novo synthesize the saturated acids such as palmitic acid, which is chain-elongated and desaturated to monounsaturated acid such as oleic acid. The crab converts the exogenous acids such as linolenic acid to the highly polyunsaturated fatty acids such as icosapentaenoic acid in the similar way found on fish.
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Fatty Acid Metabolism in Blue Crab, <I>Portunus trituberculatus</I>, with Special Reference to <I>De Novo</I> Synthesis and Conversion

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