海藻のクロロフィルに関する研究-II : TLCによるワカメのクロロティルおよびその変性化合物の分離
スポンサーリンク
概要
- 論文の詳細を見る
We used silica gel TLC of the pigments of dried ‘Wakame’ (Undaria pinnatifida) to confirm the existence of some altered chlorophyll a and chlorophyll c which stayed on the baseline. In the pigments extracted from dried ‘Wakarne’ of high quality several bluegreen spots appeared close to Rf 0.4 on silica gel plate when developed by n-hexan-diethyl ether-acetone (6:3:2, v/v). These pigments were considered to be degradation products of chlorophyll a. When the chlorophyll a fraction separated by sucrose column chromatography from whole pigments of fresh ‘Wakame’ was stored in a dark room for 2 weeks at 5°C, it produced two clear blue green spots with some faints green spots. But pigments of ashed ‘Wakarne’ (Haibashi-wakame) showed two blue-green spots, one of which shifted to Rf 0.40, and also others which were chromatographically similar to chlorophyll b of spinach pigments and shifted to a lower position on the silica gel plate. The blue and red absorption maxima of A1, B3, and C2 pigments were in the range of 427-430nm and 661-66.2nm in diethyl ether. The blue/red absorbance ratios in pigments A1 and C1 (l.45 and 1.35) were larger than in B3 (1.30) and common chlorophyll a (1.29-1.30). These pigments were confirmed to be phytol; their IR spectra absorptions appeared near 1740, 1700, and 1650 cm-1 in CCl4. From these results it was deduced that A1, B3, and C1 pigments were similar to the chlorophyll a contained in spinach and other plants. The pigments B1 and B2 did not contain phytol when hydrolysed. It seems that the pigment B1 was the Mg-free derivative because the absorption near 1650 crn-1 in the IR spectra in CCl4 was not present. The absorption spectra of pigment B4 differed from pigments B1-B3 considerably, shifting to 410nm on the blue and 655nm on the red and having a blue/red absorbance ratio of 3.15. The IR spectra of pigment B4 was similar to C2 in CCl4; a prominent absorption near 1740cm-1 and a weak absorption near 1650cm-1 were observed, but the one near 1710cm-1 was not seen. The absorption maxima (666, 410nm) of pigment B5 appeared as a grey spot on the silica gel plate (Rf 0.58). This result was similar to that for pheophytin a (666, 409nm), although the blue/red absorbance ratio (2.14) was higher than for pheophytin a (2.03-2.08).
- 公益社団法人 日本水産学会の論文
著者
関連論文
- 魚肉のイノシン分解酵素におけるニ,三の知見
- 海藻の色
- ゴウシュウマダイ筋肉5-ヌクレオチダーゼの精製と性質
- 海藻のクロロフィルに関する研究-II : TLCによるワカメのクロロティルおよびその変性化合物の分離
- 海藻のクロロフィルに関する研究-I : 乾燥ワカメ抽出液の吸収スペクトルとクロロフィル含量
- Solubilization and Isolation of Chlorophyll-Protein Complexes from Hizikia fusiformis.
- 褐藻ヒジキのクロロフィルタンパク質複合体の色素組成と色素の季節的変動〔英文〕
- ヒジキのクロロフィル-タンパク質複合体の分離と色素組成
- 海藻のクロロフィルに関する研究-3-褐藻類のクロロフィルCについて
- ゴウシュウマダイのATP関連物質の消長とイノシン酸分解酵素活性