MycobacteriumとNocardiaの色素の比較

概要

論文の詳細を見る
Carotenoid pigments were extracted from 20 strains of various chromogenic mycobacteria by the method previously described (Tsukamura, M.: J. Biochem. (Tokyo), 51: 169, 1962) (Table 1). The epiphasic pigments were dissolved in petroleum ether and passed through a column of activated alumina. A major portion of pigments appeared as a yellow band in a column. The pigment was readily eluted into petroleum ether from a pigmented portion of the column. The pigment showed absorption maxima at 425, 450 and 475 mμ in petroleum ether or in n-hexane. These absorption maxima were identical as those of beta-carotene (Table 1). Caro t enoid pigments of pigmented nocardia also were extracted from 10 strains by a similar method. Unlike the pigments of mycobacteria, however epiphasic pigments of the nocardia could not be eluted into petroleum ether after absorption to alumina column but could be eluted into ethanol. The ethanol solution of pigments was evaporated under reduced pressure and then the pigments were dissolved in petroelum ether or in n-hexane before measurement of their absorption maxima. A major portion of carotenoids of the nocardia appeared as a broad reddish band in a column and showed absorption maxima at 435, 458 (to 460) and 488 mμ in petroleum ether or in n-hexane. These absorption maxima resembled those of gamma-carotene but differed slightly. All 10 strains showed a major pigment of the same nature (Table 2). It was remarkable that, inspite of difference in species, all 20 strains of mycobacteria or all 10 strains of nocardia showed the same main pigment within the genus, respectively (Fig.1).
日本結核病学会の論文

<I>Mycobacterium</I>と<I>Nocardia</I>の色素の比較

スポンサーリンク

概要

著者

関連論文

スポンサーリンク