肺病巣から分離したナイアシンテスト陽性のMycobacterium auium-Mycobacterium intracellulare complex について

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Mycobacterium avium-M. intracellulare complex opportunely causes human pulmonary disease. This species generally gives a negative reaction to the niacin test. The present paper describes the isolation of a strain of niacin-positive M. avium-M. intracellulare complex from lung lesions of a patient. The characteristics of the isolate are shown in Table 1. The niacin test was performed by the qualitative and the quantitative methods which were slightly modified from the original description by Konno. In the qualitative method, the positive reaction to the niacin test, i.e., pink coloring, was observed in the isolated strain. In the quantitative method, the organism was incubated on the synthetic liquid Sauton medium for three weeks. After incubation, the liquid medium was kept at 100°C for one hour, and then filtrated through the Toyo filter paper. The bacilli were dried and weighed. The volume of the filtrate was measured, and a 2 ml sample of the filtrate was mixed with 1 ml of 10 per cent cyanogen bromide solution and 1 ml of ammonium-buffer solution. The reaction color was measured spectrophotometrically at 405 mμ. These tests were performed simultaneously to the isolated strain, M. tuberculosis H37Rv and M. intracellulare ATCC 15984. The results are shown in Figure 1. Whereas the niacin production of M. intracellulare ATCC 15984 was less than 1.0μg per 1 mg of the dried bacilli, the isolated strain produced about 3 μg and H37Rv produced about 8 μg. This quantitative method showed that the isolated strain produced apparently more niacin than M. intracellulare.
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肺病巣から分離したナイアシンテスト陽性の<I>Mycobacterium auium-Mycobacterium intracellulare</I> complex について

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