抗結核剤の結核菌発育遅延作用の比較

概要

論文の詳細を見る
The relationship between the growth rate of tubercle bacilli and the concentration of antituberculous agents was studied. The growth rate was observed by two methods: First, the ratio of the timeof appearance of visible colonies on a medium, to which 20 to 50 viable organisms were inoculated, taking the time on control medium as 1 (Tsukamura & Noda: Med. & Biol., 45: 150, 1957) (The appearance of colonies occurred after 13.8-1.0 days, when inoculated 33.6-6.3 viable organisms); second, the generation time measured by the method of Youmans and Youmans (J. Bacteriol., 58: 247, 1949) (Ogawa egg medium was used). The test organism was Mycobacterium tuberculosis H37Rv and the Ogawa egg medium was usedthroughout. After homogenizing by shaking with glass beads, the bacteria were suspended in saline anda bacterial suspension, 10mg wet weight/ml, was prepared. This suspension and its dilutions, 10-1to 10-5, were used as the source of inoculation. The inoculation was done using a spiral loop, whichdelivers a 0.02ml amount (Tsukamura & Noda: Kekkaku, 32: 639, 1957). The tubes inoculatedwere stoppered by a gum cap with a pin hole and incubated at 37°, and the growth of colonies wasobserved every day. Antituberculous agents were added to the medium before sterilization. Themedium was poured at 7ml quantities into tubes, 165 by 16.5mm, and was made as slopes by sterilization at 90° for 60 minutes. The results are shown in Fig. 1 and 2. The degradation of antituberculous agents in mediumduring the incubation at 37° was tested as shown in Table 1. It was shown that, out of the ten agentstested, only rifampicin and ethionamide are degraded during the incubation. The degradation ofrifampicin was more marked than that of ethionamide. The results showed necessity of modificationof curves in cases of these two agents. It was suggested that, after such modification, the curve ofrifampicin be similar to a straight line. From the results obtained, the antituberculous agents could be classified into three groups. Group I. The agents which belong to this group showed a straight line-relationship betweenthe growth rate of tubercle bacilli and the concentration of an agent. Increase of the concentration of the agents in suggested to produce increased activity in lesions, showing a straight line-relationship. The following agents belong to this group: Streptomycin, kanamycin, p-aminosalicylate, ethambutol, rifampicin, cycloserine, and amithiozone. Group II. Capreomycin and ethionamide belong to this group. Increase of the concentrationis accompanied by marked increase of activity. The increase of the activity is suggested to be higherthan expected from the straight line-relationship. It is also possible that decrease of the concentration may cause a marked decrease of the activity. It is desired to make efforts to elevate the concentration of these agents in lesions. Group III. Only isoniazid belongs to this group. This agent does show no activity if the concentration is lower than its critical concentration, but it shows almost complete inhibition of the growthwhen the concentration reaches the critical concentration. The use of higher concentrations thanthe critical one will not give mich effectiveness. Use of a large dose of this agent will have only meaning to maintain effective concentration for a longer period.
日本結核病学会の論文

抗結核剤の結核菌発育遅延作用の比較

スポンサーリンク

概要

著者

関連論文

スポンサーリンク