菌体を抗原としたELISA法によるA群レンサ球菌C-多糖体抗体の測定

概要

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The present paper describes the measurement by ELISA of antibody against group A streptococcal specific C-polysaccharide in the immunized rabbit and human sera with trypsinpronase treated whole cells as antigen.The optimal concentration of the enzyme-treated whole cells for coating to wells was 2×107cells/well. Absorption tests with whole cells of group A streptococcus, purified C-polysaccharide and N-acetylglucosamine indicated that the antibody specific to C-polysaccharide was detected by ELISA. There was a highly significant correlation between the anti-C-polysaccharide antibody titrated with enzyme-treated whole cells and that with purified C-polysaccharide as antigen. When the antibody titers (IgG class) against the enzyme-treated whole cells of the sera of patients with acute post-streptococcal glomerulonephritis and rheumatic fever were compared with those of the sera of healthy individuals by ELISA, the former was significantly higher than the latter.The results suggest that anti-C-polysaccharide antibody can be measured by ELISA with enzyme-treated whole cells as antigen.
日本細菌学会の論文