高速液体クロマトグラフィーによる米ぬか油中のフェルラ酸エステルの定量

概要

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An ultraviolet absorption spectroscopic method has been used generally for the determination of ferulate content in rice bran oil. However, the analytical accurate results are not obtainable, because of the presence of some substances interfering the above method. Moreover, another method using both TLC and ultraviolet spectroscopy takes a long time for the analysis. <BR>In this work, the quantitative estimation of ferulates in rice bran oil by high performance liquid chromatography (HPLC) was investigated. It was found that operating conditions shown in Table-2, were optimum for the separation of ferulate from other components in rice bran oil. The peak of separated ferulate in the chromatogram was measured, and the calibration curve of the relation between the weight of ferulate and peak area was used to calculate the f erulate content. In this case, β-sitosteryl ferulate was used as the external standard sample. <BR>Since the interfering substances for the ultraviolet spectroscopy were removed and the ferulates were separated by HPLC the results acquired by the method mentioned above are more accurate as compared with that were determined directly by ultraviolet spectroscopy. In addition, the analytical procedure takes less than 15 minutes in almost cases. It is apparent that the time needed for the analysis per one sample by HPLC is shorter than that by the method using both TLC and ultraviolet spectroscopy.
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