大豆油脂肪酸メチルのガスクロマトグラフィーにおける問題点

概要

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Some fundamental factors influencing on the gas chromatographic analysis of the fatty acid composition of soybean oil were studied. A gas chromatograph equipped with a flame ionization detector and an integrator was used. Three glass columns were prepared which were packed respectively with 7.5%, 10% and 20% of diethylene glycol succinate on 100200 mesh Chromosorb W AW DMCS. The bleeding of the liquid phase was negligible during the operation for 185hr on 7.5% column and 200hr on 10% and 20% columns. Of the three columns, 20% DEGS column was found most suitable for the analysis of fatty acid composition of soybean oil. Relative mass response factors of methyl linoleate and linolenate to methyl stearate were 1.00 when 20% DEGS column was used. It was also found that the proper sample size of 1 or 2% (wt/vol) n-hexane solution of a methyl ester mixture from soybean oil for the gas chromatographic analysis is 2μl.
社団法人日本油化学会の論文