油脂の簡易分析法としての円形ペーパークロマトグラフィー

概要

論文の詳細を見る
A simple and rapid method for the separation and identification of fatty acids and glycerides has been developed by using circular paper chromatography. Petroleum hydrocarbon, dodecylbenzene, and tetralin were used as the stationary solvents. As the developing solvents, 90% acetic acid and methanol-acetic acid, both saturated with a stationary solvent, were suitable for the separations of fatty acids and of glycerides respectively. The fatty acids were detected on paper by successive treatment with 0.5% lead acetate, water, and ammonium sulfide solution. The selective detection of unsaturated acids by spraying with a 2% solution of iodine in ethanol was also applied. The separation of various “critical partners” of fatty acids was achieved by the method of double development, involving the first low-temperature development with petroleum ether under dry ice and the second reversed-phase development with the usual solvent systems at room temperature. The unsaturated glycerides were chromatographed as their mercuric acetate addition compounds, and were detected by spraying with a 0.2% solution of diphenylcarbazone in ethanol or by soaking in a Sudan black solution. The clear-cut separations of fatty acids and glycerides into their homologs can be attained by these circular chromatographic techniques, since the separation of unsaturated triglycerides mainly depends on their unsaturation and that of mono-, di-, and triglycerides mainly depends on the number of their free hydroxyl groups. This method was successfully applied to the analysis of various fats, fatty acids, and their products, and was confirmed to be of great value as a simple, rapid, and practical method for their analysis.
社団法人日本油化学会の論文

油脂の簡易分析法としての円形ペーパークロマトグラフィー

スポンサーリンク

概要

著者

関連論文

スポンサーリンク