数種食品防腐剤の嫌気性菌に対する発育抑制効果と培地成分の影響

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Effect of components of culture media for anerobic bacteria on the sensitivity of various Clostridia to chlortetracycline (CTC), tylosin (TI), furyl-furamide (FF) and nitrofurazone (NFS) have been examined. Test organisms were 9 strains of stock Clostridia including Clostridium perfringens, Cl. tetani, Cl. histolyticum, Cl. sporogenes and 3 types of Cl. botulinum, in addition, 10 strains of Clostridia isolated from fish sausages and fish hams, species names of which were shown in Table 2. Culture media employed in this experiment were the thioglycollate medium (TGC), the brain heart infusion medium containing 0.1% agar (BHI+A), and the BHI medium covered with sterile liquid paraffin (BHI+P) which were adjusted to pH 7. Minimal inhibitory concentrations (MIC) of test drugs were determined in the respective medium containing binary dilution series of each drug after being incubated at 37°C for 24, 48, 72 and 96 hours. Results obtained may be summarized as follows: 1. As to CTC, no significant difference in MIC values was observed according to the kind of culture medium, while these values varied markedly by the length of incubation period which might be due to the instability of CTC in the culture media (Figs. 1 and 2). 2. In the case of TI, much higher MIC values were noted in the TGC medium than those obtained in both of the BHI media in spite of the stability of the drug in the TGC medium, whereas no appreciable change could be seen depending upon the length of incubation period (Table 1). 3. In the TGC medium, FF gave highest MIC values (Table 1), in addition, these values increased greatly according as the incubation period increased. These phenomena might be due to rapid inactivation of the drug by the action of reducing agents in the medium (Fig. 2). To the contrary, MIC values were fairly low when determined in either BHI-A or BHI-P medium, moreover, almost no appreciable change was noted by the length of incubation period (Tables 1 and 2). 4. Growth inhibitory effect of NFS to various Clostridia was found to be the lowest among 4 drugs so far tested, especially almost no activity was observed in the TGC medium which might be explained by the rapid destruction of the drug in the medium during incubation (Fig. 2).
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数種食品防腐剤の嫌気性菌に対する発育抑制効果と培地成分の影響

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