Determination of the immunoreactive fraction of radiolabeled monoclonal antibody and F(ab')2 fragment against a human renal cell carcinoma cell line.

概要

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In order to examine the immunological avidity of F (ab')2 fragment of monoclonal antibody generated against a human renal cell carcinoma cell line (C5H), a binding assay was performed against human renal cell carcinoma cell lines using iodinated intact C5H and it's F(ab')2 fragment. Stable production of F(ab')2 fragments were obtained by pepsin digestion at pH3.5. The fragments were purified by affinity and gel-filtration chromatography in acceptable yield. This binding assay indicates that F(ab')2 fragments retain almost the same immunological fraction (51.2%) and avidity (4.7×105/cell) as the intact antibody (49.9%, 5.2×105/cell), even after the enzyme digestion and iodinating procedures.Based on these findings, it was concluded that the iodinated F(ab')2 fragments can be applied for further in vivo radioimmunodetection assay.
日本臨床免疫学会の論文