THE MEASUREMENT OF DNA-ANTI-DNA COMPLEXES BY FLUOROPHOTOMETRY

概要

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In this study, we tried to detect DNA-anti-DNA complexes, which were isolated from SLE sera by 3.5% PEG precipitation, by fluorophotometry using DABA.In 22 of 31 sera from patients with active SLE and in 9 of 48 sera from patients with inactive SLE, DNA-anti-DNA complexes were found. The amount of DNA in the immune complexes in sera of SLE with active disease (4.43±2.10μg/ml) was significantly greater than that of SLE with inactive disease (1.20±1.15μg/ml, P<0.01). In addition, that of SLE correlated with the titer of anti-DNA antibodies and serum complement activity, but had no relationship with proteinuria. On the clinical coarse, that decreased significantly after prednisolone therapy.On these results, it was suggested that the fluorophotometry was useful to detect DNA-anti-DNA complexes in sera from SLE patients.
日本臨床免疫学会の論文