A new simple plastic chemotaxis device of the Boyden chamber type utilizing an immunoassay plate.

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概要

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• A new simple and economical plastic Boyden chamber for in vitro assay of leukocytes chemotaxis was devised by the use of a commercially available immunoassay plate. Zymosan-activated rat serum, formly-methionyl-leucylphenylalanine and leukotriene B<SUB>4</SUB> caused concentration-dependent migration of the leukocytes into the lower chamber. About 50% of the cells loaded in the upper chamber migrated into the lower chamber for 80 min at the optimal concentrations of the three chemoattractants tested.

• 公益社団法人 日本薬理学会の論文

著者

• Tsurufuji Susumu

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Tohoku University

• Nakagawa Hideo

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Tohoku University

• Watanabe Kazuyoshi

  Department Of General Medicine Juntendo University School Of Medicine

• NAKAGAWA Hideo

  Department of Biochemistry Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• WATANABE Kazuyoshi

  Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University

• WATANABE Kazuyoshi

  Department of Biochemistry Faculty of Phrmaceutical Sciences, Toyama Medical and Pharmaceutical University

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