The Kinds of Carbohydrates Contained in the Tea-Seed

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The carbohydrates in the cotyledon and nutshell of the tea-seed were divided into fourteen fractions, and the free sugars and the combined sugars of each fraction have been investigated by the method of paper-chromatography. The obtained results were as follows; In the cotyledon, glucose, fructose, sucrose, maltose, raffinose, stachyose and two unknown oligosaccharides (Rf 0.09 and 0.06), and in the nutshell, a very slight quantity of fructose, sucrose and two unknown oligosaccharides (Rf 0.09 and 0.06) were detected as free sugars. As the hydrolyzates of free sugar fraction, galactose, fructose, glucose, xylose, arabinose rhamnose and galacturonic acid were found in the cotyledon, and fructose, glucose, xylose in the nutshell. Glucose, xylose, and galacturonic acid were detected in the cotyledon as the components of hot 50% ethanol-soluble polysaccharides, but not found in the nutshell. Glucose, fructose, arabinose and galacturonic acid were found in the cotyledon as the component sugars of cold water-soluble polysaccharides, but not found in the nutshell. A large amounts of glucose were detected in the cotyledon as the component sugar of hot 33% CaCl2-soluble polysaccharides (mainly starch), and xylose alone was detected intensively in the nutshell as the component of hot water-soluble polysaccharides. Glucose, galactose, arabinose and galacturonic acid were found in the cotyledon as the component sugars of hot 0.5%NH4-oxalate-soluble polysaccharides. A large quantities of glucose and fructose and a very small quantity of arabinose were detected in the cotyledon and arabinose alone in the nutshell, as the component sugars of cold 5% NaOH-soluble polysaccharides. And moreover, fructose, galacturonic acid and arabinose were found as the components of hot 5% NaOH-soluble polysaccharides, the former two in the cotyledon and the latter one in the nutshell. As the component sugars of β-and γ-cellulose fractions, fructose, glucose and xylose were detected in the cotyledon, and arabinose alone, in the nutshell. Besides the above-mentioned carbohydrates, α-cellulose and lignin were also detected both in the cotyledon and nutshell.
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The Kinds of Carbohydrates Contained in the Tea-Seed

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