やぎ第1胃液およびセルラ-ゼの牧草消化能に及ぼす可溶性シリカの影響

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To investigate the effect of soluble silica (sodium silicate, Na2SiO3) on digestion of forage with rumen fluid of goat and cellulase, an experiment measuring organic matter disappearance (OMD) of Rhodes grass (Chloris gayana) was conducted using in vitro incubation for 48hr with rumen fluid of goat and with 2.5% Onozuka cellulase SS (P-1500) in an acetate buffer (pH 4.6). In both cultures sodium silicate was added to provide 400, 800 and 1, 200ppm of silica (SiO2) prior to incubation. Rumen fluid was collected from three goats fitted with rumen fistulae and fed a hay. Incubation with cellulase was preceded by that with acid pepsin solution for 48hr. Materials were leaf and stem of an internode elongation (immature) and mature stage of regrowth. The results obtained were as follows: 1. In incubation of leaf and stem of immature and mature materials with rumen fluid containing soluble silica, OMD tended to decrease with increasing soluble silica and at 1, 200ppm level of silica OMD decreased significantly. During the incubations pH of the rumen cultures was close to the upper range of pH of rumen fluid reported. 2. In incubation of materials with cellulase solution, OMD decreased with increasing soluble silica. Although sodium silicate increased pH of the cellulase solution from 0.17 to 0.34 than that with no sodium silicate, increased pH of the solution did not affect OMD. Therefore, soluble silica depressed OMD of the materials by incubation not only with rumen fluid of goat but also with cellulase. This result suggested that cellulolytic enzyme activity was also affected by soluble silica.

やぎ第1胃液およびセルラ-ゼの牧草消化能に及ぼす可溶性シリカの影響

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