重酸素水を用いた非イオン系界面活性剤の微生物分解試験法の開発

概要

論文の詳細を見る
In order to investigate the oxidative biodegradation mechanisms of octylphenol polyethoxylate (OPEO) by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), fundamental conditions for the bacterial biodegradation test method using 18O-labelled water as an incubation medium were examined. Since 18O-labelled water is a valuable reagent, reduction of incubation medium down to 200 µL could be achieved. Furthermore, to recover OPEO degradation intermediates from a minimum amount of the medium containing basal salt, a simple solid-liquid extraction procedure was introduced. The combination of the biodegradation test using 18O-labelled water and MALDI-MS measurement thus could provide a conclusive biodegradation mechanism by the observation of incorporation of 18O atoms into the carboxyl group of OPEC during the biodegradation process.
日本質量分析学会の論文
2003-02-01