マトリックス支援レーザー脱離イオン化質量分析法による微生物の迅速識別法と微生物管理の試み(<特集>生命科学と分析化学)

概要

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Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is beginning to play an important role in a rapid discrimination of bacteria, where ribosomal proteins composed of more than 50 subunits is used as biomarkers. In this study, this technique was applied to the maintenance of bacterial culture collections. It was confirmed that most of the biomarker peaks could be observed in the whole cell lysate of model lactic acid bacteria, which was prepared simply by bead-beating and centrifugation. The mass differences of specific ribosomal subunit proteins observed on the MALDI mass spectra allowed us to discriminate two lactic acid bacteria at a subspecies level difference (Lactococcus lactis subsp. lactis and Lc. lactis subsp. cremoris). By comparing the MALDI mass spectrum of the stored culture of Lc. lactis subsp. lactis with that of the pure culture as a reference, it could be easily found that the stored culture was cross-contaminated with other lactic acid bacteria.
社団法人日本分析化学会の論文
2006-12-05