マトリックス支援レーザー脱離イオン化質量分析法による乳酸菌の迅速識別

概要

論文の詳細を見る
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied to the rapid discrimination of four lactic acid bacteria (Lactobacillu acidophilus. Lactococcus subsp. hordniae, and two strains of Lc. lactis subsp. lactis). Specific biomarker peaks generated from intact bacteria cell were observed on the mass spectra over the range m/z 4000〜12000. High reproducibility for the relative peak intensities between three culture plates under the same experimental conditions was confirmed by analysis of variance (ANOVA). The growth temperature strongly affected the peak distribution of the biomarkers, whereas the difference in the culture media (MRS and LB media) gave similar mass spectra. Under the optimized conditions, four lactic add bacteria with different genus, species, or strains could be discriminated.
社団法人日本分析化学会の論文
2004-06-05