5-Aza-2-deoxycytidine reactivates the CDH1 gene without influencing methylation of the entire CpG island or histone modification in a human cancer cell line
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概要
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It is well-recognized that DNA methylation and histone modifications play critical roles in epigenetic regulation of gene activity through the alteration of chromatin structure. Recent studies have shown that in a subset of cancer cells, the silencing of the human E-cadherin (CDH1) gene is associated with hypermethylation of the CpG island. However, the associated molecular mechanism remains unclear. To understand the mechanism, we have investigated the alteration of CpG island methylation and histone modifications during the reactivation of the CDH1 gene by treatment with 5-aza-2-deoxycytidine (5-aza-dC). Although the CDH1 gene expression was recovered by treatment with 5-aza-dC in a liver cancer cell line Li21, the methylation status of the entire CpG island and acetylation and methylation status of associated histones were not significantly altered. These results demonstrate that the silenced CDH1 gene can be reactivated without apparent alteration of histone modification or CpG island methylation. (Communicated by Takashi SUGIMURA, M.J.A.)
- 日本学士院の論文
著者
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Takeda Ken
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
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SHIRAISHI Masahiko
DNA Methylation and Genome Function Project, National Cancer Center Research Institute
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TACHIBANA Ken
DNA Methylation and Genome Function Project, National Cancer Center Research Institute
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Takeda Ken
Department Of Hygiene Chemistry Faculty Of Pharmaceutical Science Tokyo University Of Science
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TAKEDA Ken
Department of Hygiene-Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
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