Specific Methylation Status of the Entire CpG Island Is Not a Prerequisite for the Formation of an Inactive Chromatin at the Promoter Region in Cancer Cells(Communications to the Editor)
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概要
- 論文の詳細を見る
It is well-recognized that DNA methylation causes gene silencing. However, although DNA methylation is closely associated with alterations in chromatin structure, it is not clear whether methylation at a particular locus contributes to the formation of an inactive chromatin structure. In this study, we have investigated the chromatin structure of the CpG island of the human CDH1 gene in cancer cells. The CDH1-CpG island was differentially methylated between silenced cells, however, DNase I hypersensitive site that are present in the expressing cell were commonly lost in silenced cells and transcription factors were excluded from their binding sites. These results demonstrate that formation of an inactive structure was not affected by heterogeneous methylation status of the CpG island.
- 公益社団法人日本薬学会の論文
- 2003-01-01
著者
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Shiraishi Mitsuru
Medicinal Chemistry Research Laboratories Pharmaceutical Research Division Takeda Chemical Industrie
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Shiraishi Mitsuru
Pharmaceutical Research Laboratories Ii Takeda Chemical Industries Ltd.
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Shiraishi Mitsuru
Pharmaceutical Research Laboratories Ii Pharmaceutical Research Division Takeda Chemical Industries
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SHIRAISHI Masahiko
DNA Methylation and Genome Function Project, National Cancer Center Research Institute
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Sekiya T
Dna Methylation And Genome Function Project National Cancer Center Research Institute
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Koizume Shiro
Dna Methylation And Genome Function Project National Cancer Center Research Institute
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Shiraishi M
Medicinal Chemistry Research Laboratories Pharmaceutical Research Division Takeda Chemical Industrie
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Sekiya Takao
Dna Methylation And Genome Function Project National Cancer Center Research Institute
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