Gene Transfection into HeLa Cells by Vesicles Containing Cationic Peptide Lipid
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概要
- 論文の詳細を見る
Lipid vesicles are potentially useful as microcapsules for drug and/or gene delivery. We developed cationic lipid vesicles consisting mainly of sorbitan monooleate (Span 80) and cationic peptide lipid (CPL), and evaluated the CPL vesicles as gene transfection vectors. The optimum CPL concentration for gene transfection into HeLa cells was found to be 20 wt % of total lipid, and such CPL vesicles did not exhibit significant cytotoxicity. Co-culture of Poly-L-lysine and plasmids prior to making CPL vesicle-plasmid complexes was effective. Lipofection using LipofectAMINE was suppressed in 10% serum-supplemented medium. The transfection efficiency of 20 wt % CPL vesicles, however, was not affected by serum in the medium when plasmids were treated with poly-L-lysine.
- 社団法人 日本農芸化学会の論文
著者
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SUGAHARA Takuya
Faculty of Agriculture, Ehime University
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TAKASHIMA Shigemitsu
Department of Clinical Research and Surgery, Shikoku Cancer Center
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Sugahara Takuya
Faculty Of Agriculture Ehime University
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Takashima Shigemitsu
Department Of Clinical Research And Surgery National Shikoku Cancer Center
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HEIKE Yuji
Department of Medical Oncology and Clinical Research National Shikoku Cancer Center
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HOSOKAWA Mami
Department of Biology and Earth Sciences, Faculty of Science, Ehime University
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Hosokawa Mami
Department Of Biology And Earth Sciences Faculty Of Science Ehime University
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Yoshimura Norikazu
Faculty of Pharmaceutical Sciences, Hokuriku University
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OHAMA Yumi
Faculty of Agriculture, Ehime University
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SAKATA Kozue
Faculty of Agriculture, Ehime University
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HISAEDA Yoshio
Faculty of Engineering, Kyushu University
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KATO Keiichi
Faculty of Engineering, Ehime University
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Takashima Shigemitsu
Department Of Breast Oncology National Hospital Organization Shikoku Cancer Center
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YOSHIMURA Norikazu
Faculty of Engineering, Ehime University
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TAKASHIMA Shigemitsu
Department of Medical Oncology and Clinical Research, National Shikoku Cancer Center
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HOSOKAWA Mami
Department of Medical Oncology and Clinical Research, National Shikoku Cancer Center
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HEIKE Yuji
Department of Medical Oncology and Clinical Research, National Shikoku Cancer Center
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