Purification and Characterization of Carboxyl Proteinase from Aspergillus kawachii
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概要
- 論文の詳細を見る
A carboxyl proteinase was purified from rice koji of Aspergillus kawachii to a homogeneous state on polyacrylamide gel electrophoresis. The molecular weight and isoelectric point of the enzyme were 35, 000 and 3.9, respectively. The enzyme was most active between pH 2.8 and 3.4 with hemoglobin as substrate, and stable in the pH range of 2.2-6.4. The optimum temperature of the enzyme reaction was at 50°C. The serine content was highest and no methionine was found in the enzyme. The enzyme was inhibited by typical inhibitors for carboxyl proteinases such as DAN, EPNP, and SPI.
- 社団法人 日本農芸化学会の論文
著者
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YAGI Fumio
Laboratory of Biochemistry and Nutritional Chemistry
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Tadera Kenjiro
Laboratory Of Biochemistry And Mutritional Chemistry
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Kobayashi Akira
Laboratory Of Biochemistry
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FAN Jianqiang
Laboratory of Biochemistry and Nutritional Chemistry, Faculty of Agriculture, Kagoshima University
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