魚類のイクチオフォヌス症に関する研究-5-培養

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Authors observed the shapes of ichthyophonus cultured with 0.85% saline and artificial medium ; Sabouraud dextrose agar and TGC fluid medium adding 10% calf serum. Pieces of visceral organs of affected yellowtail fry were kept in 0.85% saline at 5℃ for one month. Various sizes of multinucleate spherical bodies germinated three days after inoculation. Endospores were produced in one week after inoculation but they never grew during the one month observation. On the Sabouraud dextrose agar long hyphae grew from pieces of visceral organs of affected Japanese eel at 18℃ in one week after inoculation. Heavy-walled spores were formed at the tips of the hyphae in one week after that. In the TGC fluid medium, hyphae grew from pieces of visceral organs of affected yellowtail fry at 23℃ in one week after inoculation. Hyphae grew making many branches and hyphal bodies at the tips of the hyphae during four months observation.1) ブリ稚魚とニホソウナギに感染していたイクチオフォヌスを用いて, 宿主の死後の形態, サブ口ー寒天平板と10%仔牛血清添加TGC培地での培養下での形態を観察した。 2) 宿主の死後, あらゆる段階の多核球状体が発芽し, 内生胞子がつくられた。 3）サブ口ー寒天平板上ではheavy-walled spore がつくられた。 4) 10%仔牛血清添加TGC培地では多数の分枝をもつ菌糸が増殖した。
三重大学水産学部の論文