細胞接着状態での凍結保存に適したDimethyl sulfoxide濃度の検討

概要

論文の詳細を見る
Cells have high molecular discerning ability. Taking advantage of this ability, development of cell-adhesion systems attached to matrices has been attempted for their use in the field of biosensors. For the repeated use of biosensors, preservation of the cells bound to matrices is quite necessary. Dimethyl sulfoxide (DMSO), concentration of which is 5-10v/v%, is often used as a cryoprotective agent for animal cells. In the present study, we investigated cryopreservation of HepG2 cells in the cell-adhesion state attached to a glass matrix by applying higher concentrations of DMSO than those used in the suspension state. The rate of cell-adhesion increased, as a result, with augmentation of DMSO concentration. Cell viability also became higher with the increase in the concentration up to 20v/v% of DMSO. However, it decreased with further increase in the concentration. Then, it can be concluded that the application of 20v/v% DMSO is appropriate for cryopreservation of cells in the cell-adhesion state.
低温生物工学会の論文
2013-10-15