MNM-4B-1 ハイブリッド顕微鏡内ナノマニピュレーションシステムによるモデル生物解析(セッション 4B マイクロ・ナノ技術によるロボティクス・メカトロニクスの新展開)

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This paper presents a novel biological specimen analysis system by hybrid microscope, which is combined optical microscope and environmental-scanning electron microscope (E-SEM) based on nanorobotic manipulation system. The E-SEM provides us the high resolution image of specimen with water containing condition by specially built detector and temperature controlling stage. However the E-SEM image, especially SEM image, is surface image, hence it is impossible to obtain the inner structural imaging which is important for biological analysis. In this work, we combine the optical microscope (OM) and E-SEM to realize biological specimen analysis by optical microscope image including fluorescent imaging, and nano-scale manipulation by E-SEM imaging. In this paper, we observe C. elegans as biological specimen analysis. The viability analysis has been done from the eGFP fluorescent intensity of C. elegans under E-SEM observation and electron beam irradiation. This system will be applied as future nano-surgery system based on nanomanipulation system for biological specimen.
一般社団法人日本機械学会の論文
2010-10-12