2A1-J01 電気浸透現象を利用した微少流体の局所噴出制御

概要

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This paper presents the local spout of biological sample method with using the electro-osmotic phenomenon by a pipette manipulation. The pipettes can be used to control the local environment around/inside single cells. Conventionally, the microinjection with pipette has been conducted by air pressures. By this method, the spout of high viscosity solutions is difficult caused by the frictional forces between the surface of a pipette and a solution. In this paper, the spouting method using electro-osmotic phenomenon is used. It is considered that the high viscosity solution can be spouted by the direct forces applied to the molecules in solution. In this research, liposomes are fabricated from an oil/water interface for the investigation of the reaction of biological reagents. And the amount of spout solution in this method is investigated by injection of fluorescent solution into a phospholipid-coated microdroplet.
一般社団法人日本機械学会の論文
2009-05-25