1509 制限ナノ空間における長鎖DNAの電気泳動現象(OS15-2 先端的熱流体計測法,オーガナイズドセッション)

概要

論文の詳細を見る
In this paper, we report flow dynamics of λDNAs in nanochannels under DC electric fields. λDNAs which are labeled by a fluorescent dye are suspended in an electrolyte solution and injected into nanochannels which have different depths ranging from 330 nm to 650 nm. By applying DC electric field in the nanochannels, λDNAs flow due to electrokinetic phenomena, such as electrophoresis of λDNAs and electroosmosis flows of the electrolyte solution. The experimental results reveal that the velocity of λDNAs decreases as the degree of the confinement increases in the shallower nanochannels. A simple theoretical model to predict the flow velocity of λDNAs is developed by taking into account effects of confinement due to the nanochannels. The model is developed based on Langevin equation which includes forces due to electrophoresis and electroosmosis. The validity of the model is discussed by comparing a coefficient for the degree of the confinement with those experimentally obtained from the electrophoretic mobility and the diffusion coefficient.
2010-10-29