T0501-5-4 制限ナノ空間におけるDNAの流動計測とそのモデル化([T0501-5]マイクロ・ナノスケールの熱流体現象(5))

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In this paper, we report the flow dynamics of,DNA under the electric field in a nanochannel. The λDNA labeled by YO-PRO-1 is introduced in nanochannels with a depth of 330, 430 and 650 nm. Applying DC electric field, λDNA is forced to flow due to electrokinetic phenomena, i.e. electrophoresis and electroosmosis flow. The flow is successfully visualized and observed by a fluorescent microscope. The dynamical behavior of λDNA is measured at various electric fields, where the velocity linearly increases with the electric field. It is found from the experimental results that the velocity is smaller than that in bulk solutions. The confinement effect of nanochannel can be seen because the velocity increases as the channel depth. Furthermore, a simple theoretical model is developed to explain the effect of the confinement. The comparison between experiment and theory reveals that the decrease of the velocity may be mainly caused by the increase of the viscous effect due to the confinement of the nanochannel wall.
2010-09-04