743 電場影響下におけるDNA断片の流動に関する動力学解析(OS21.細胞・生体分子の計算バイオメカニクス(1),オーガナイズドセッション)

概要

論文の詳細を見る
Denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are well-used techniques in order to analyze base sequences of DNA. One base resolution technique attracts much attention not only from scientific viewpoints but also from clinical aspects. We have developed simulation techniques focusing on fluid dynamics of DNA fragments in solvents using molecular dynamics procedures. Purposes of our study are analysis of the behavior of DNA molecules during electrophoresis and suggestion of a way to artificially control DNAs. In this study, molecular dynamics simulations for DNA fragments under external electric fields are carried out. Under the homogeneous electric field at 1.0 × 10^<-2> V/m, both models of 10 bp poly(dA)-poly(dT) and 10 bp poly(dG)-poly(dC) DNAs are accelerated against the field direction because of their negative charges. During the simulations, DNA fragments conserve their double strand structures in water solvent and any melting in both models is not observed within 1.0 ns.
一般社団法人日本機械学会の論文
2008-11-01