T0502-3-5 DNA断片における拡散係数の全原子MD解析(マイクロ・ナノ領域におけるバイオ・熱流体の融合学術創成(3))

概要

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In recent years, it has been clarified that single nucleotide polymorphism (SNP) is related to the incidence of cancer and hereditary disease. Therefore, the rapid distinction of SNPs in DNA sequences is important. Denaturing Gradient Gel Electrophoresis (DGGE) method is one of widely used techniques to identify SNPs. In DGGE, differences in sequences influence the displacements of DNA fragments. However, the detailed flow dynamics in DGGE are not clarified fully. In this study, we investigate the dynamics of DNA fragments in solvent medium. Here, we analyze the flow of 149 bp DNA with generalized Born (GB) model to get correspondence with the experimental data. As a result, we efficiently estimate the diffusion coefficients of 149 bp DNA and obtain highly accurate results. In addition, we discuss the relation between the collision coefficient y and the diffusion coefficient D in our dynamical computation.
一般社団法人日本機械学会の論文
2009-09-12