T0502-3-2 ナノ流路における生体高分子流動の可視化観察(マイクロ・ナノ領域におけるバイオ・熱流体の融合学術創成(3))

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In this study, we report the flow dynamics of λDNA in a nanochannel under the electric field. The λDNA labeled by YO-PRO-1 is introduced in a nanochannel with a depth of 200 nm. Applying electric field, the flow of λDNA is successfully observed by a fluorescent microscope. The velocity of λDNA is measured at various electric fields, where the velocity linearly increases with the electric field. The diffusion coefficient of λDNA is obtained from the distribution of the velocity data to be 1.04×10^<13>m^2/s, where the value is 1/4 of that in the bulk. This result indicates that the diffusion coefficient is decreased due to the confinement of nanochannel. The result obtained here can provide important informations to design nanobio-devices using nanochannels.
社団法人日本機械学会の論文
2009-09-12