J0207-2-1 電気浸透流を利用した異種細胞配置用デバイス開発および細胞間相互作用の開発([J0207-2]医療・福祉工学のための3次元造形)

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This paper presents a novel technique of positioning heterotypic cells using electroosmotic flow (EOF). Positioning heterotypic cells is a key technique toward the development of single cell analysis. Several techniques for positioning cells have been developed, such as using dielectrophoresis and physical trapping in microchannel. These techniques, however, require additional methods for subsequent biological experiments after arraying cells. In this study, we demonstrated the positioning of two kinds of cells at designated pocket-like microstructures. In this method, cells were positioned using multi-directional EOF in open-to-air flow chamber which would allow easy handling for further biological experiments. After positioning heterotypic cells, we cultivated them and observed cell-cell interactions via gap junctions by the transfer of a fluorescent dye.
2010-09-04