S0201-3-5 マイクロパターニング技術を用いた細胞伸展ダイナミクスの評価(マイクロ・ナノバイオメカニクス:細胞生物学への接近(3)界面と接着)

概要

論文の詳細を見る
Actin polymerization-mediated cell protrusion is closely associated with attachment to the substrate and constitutes an important initial step during active cell migration. However, the influence of substrate adhesion property on this fundamental process is less investigated and understood. In order to determine the effect of cell-substrate interaction on cell protrusion dynamics, this study investigated the migration behavior of fish keratocytes on a fabricated glass-substrate consisting of arrays of rectangular fibronectin patterns interleaved with plasma-treated, PLL-g-PEG-coated non-adhesive gaps. We show that both pattern size and interleaving gap length are important limiting factors on lamellipodial protrusion and subsequent migration, and that actomyosin contractility is necessary for stabilizing the lamellipodial actin network for protrusion over the interleaving gaps.
社団法人日本機械学会の論文
2009-09-12