マイクロニードル押し込みに伴う骨芽細胞の局所変形とカルシウム濃度上昇の同時観察(S02-3 生体組織のマルチスコープメカニクス(3).細部のマルチモーダル・メカニクス,S02 生体組織のマルチスコープメカニクス)

概要

論文の詳細を見る
Many studies have reported that osteoblastic cells can sense the mechanical stimuli that result in rise in the intracellular calcium ion concentration as one of the cellular responses. However, the mechanical conditions, such as stress or strain, that induce the calcium signaling response are not clearly understood yet. In this study, we prepared osteoblastic cells with labelled cellular membrane and intracellular calcium ion using two fluorescence indicator, dyes. Localized deformation was applied to a single cell by direct indentation of a glass microneedle. Subsequently, initiation of calcium signaling response and deformation of cellular membrane were simultaneously observed. Obtained results suggested that the initiating point of localized calcium signaling response was located at the front area of the microneedle tip sliding. In the surrounding area of the needle tip, it could be imagined that there was localized and complicated strain distribution. Therefore, as a future study, the experiment with improved time- and spatial-resolutions is necessary.
社団法人日本機械学会の論文
2004-09-04