T0502-2-1 マイクロ流路中におけるナノ粒子挙動の測定と制御(マイクロ・ナノ領域におけるバイオ・熱流体の融合学術創成(2)マイクロ・ナノフルイズ)

概要

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Micro biochemical analysis chip have attracted attention for merits such as speed up and high efficiency of analysis. For detection, we often use immune reaction such as antibody-antigen reaction which occurs near the wall. Therefore, the concentration distribution of biomaterials near wall greatly affects detection and reaction. In this study, we attempted to evaluate interaction between the nanoparticles and the wall and to control particles for the aim of advancement of reaction efficiency. First, we measured the concentration distribution of particles 100 nm in diameter by using TERFM which expose evanescent light which intensity decay exponentially. Using this characteristic, we identified particle's 3D-position from luminance of particle. The result shows that concentration of particles drastically increases with increasing distance from the wall. Subsequently, we tried to gather particles to the wall by applying electric field to suspension. The result shows that particles moved and attached to the wall.
2009-09-12