PAD1 and FDC1 are essential for the decarboxylation of phenylacrylic acids in Saccharomyces cerevisiae(BREWING AND FOOD TECHNOLOGY)
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概要
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The volatile phenols, to which Saccharomyces cerevisiae converts from phenylacrylic acids including ferulic acid, p-coumaric acid, and cinnamic acid, generate off-flavors in alcoholic beverages such as beer and wine. Using gene disruptants, transformants and cell-free extracts of these strains, we have verified that the adjacent PAD1 (phenylacrylic acid decarboxylase, YDR538W) and FDC1 (ferulic acid decarboxylase, YDR539W) genes are essential for the decarboxylation of phenylacrylic acids in S. cerevisiae. Pad1p and Fdc1p are homologous with UbiX and UbiD, respectively, in the ubiquinone synthetic pathway of Escherichia coll. However, ubiquinone was detected quantitatively in all of the yeast single-deletion mutants, Δpad1, Δfdc1, and double-deletion mutant, Δpad1Δfdc1.
著者
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Mukai Nobuhiko
National Research Institute of Brewing
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Masaki Kazuo
National Research Institute of Brewing
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Fujii Tsutomu
National Research Institute of Brewing
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Kawamukai Makoto
Faculty of Life Science and Environmental Science, Shimane University
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Iefuji Haruyuki
National Research Institute of Brewing
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正木 和夫
酒総研
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正木 和夫
独立行政法人酒類総合研究所
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正木 和夫
北大・理・生物科学
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正木 和夫
(独)酒類総合研究所醸造技術応用研究部門
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正木 和夫
醸造技術応用研究部門
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Masaki Kazuo
National Res. Inst. Of Brewing 3-7-1 Kagamiyama Higashihiroshima Hiroshima 739-0046 Jpn
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Fujii Tsutomu
National Research Institute Of Brewing:graduate School Of Biosphere Sciences Hiroshima University
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Iefuji Haruyuki
National Res. Inst. Of Brewing
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Iefuji Haruyuki
National Research Institute Of Brewing:graduate School Of Biosphere Sciences Hiroshima University
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Mukai Nobuhiko
National Research Institute Of Brewing:kumamoto Regional Taxation Bureau
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Kawamukai Makoto
Faculty Of Life Science And Environmental Science Shimane University
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Kawamukai Makoto
Faculty Of Life And Environmental Science Shimane University
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Iefuji Haruyuki
Graduate School Of Biosphere Sci. Hiroshima Univ.
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正木 和夫
広島大院・生物圏:酒総研
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