4. CRYO-SEM,フリーズ・レプリカによる凍結傷害発生機構の研究(平成元年度第35回凍結及び乾燥研究会研究報告)

概要

論文の詳細を見る
It has been suggested that cellular deformation which results in direct membrane-to-membrane contact is responsible for the occurrence of slow freezing injury in tertiary hyphae of mushrooms. Present study examined the quantitative relation between deformed cell number which was observed by Cryo-scanning electron microscope and intramembrane particle aggregated cell number which was observed by freeze-replicas. The observations by both Cryo-SEM and freeze-replicas were done on same samples by using JSM-840A Cryo-system. The results showed a close quatntitative relation between the number of deformed cells and the number of IMP aggregated cells. This result provides a strong evidence that cellular deformation by slow freezing causes IMP aggregation and consequently injury takes place.
低温生物工学会の論文
1989-09-30