3. ガラス状凍結保存のメカニズムに関する研究 : 原形質膜の微細構造(平成3年度第37回凍結及び乾燥研究会研究報告)

概要

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The treatment of nonacclimated (NAC) rye protoplasts with vitrification solution (VS) consisting of 7M EG, 30%(w/v) sorbitol and 6%(w/v) BSA in buffer solution, produced the distinct plasma membrane ultrastructural changes, including, formation of aparticular domains, lamellar-tohexagonal_<II>(L-to-H_<II>) phase transitions and membrane vesiculations. Loading NAC protoplasts with 1.5M EG reduced VS-induced formation of aparticular domains and L-to-H_<II> phase transitions, but not membrane vesiculations. Cold acclimation (ACC) of rye protoplasts also reduced VS-induced formation of aparticular domains and L-to-H_<II> phase transitions, but not membrane vesiculations. The treatment of these protoplasts with hypertonic solution (13S) consisting of 60% (w/v) sorbitol brought about similar effects with VS, but did not produce membrane vesiculations. It was suggested that while formation of aparticular domains and L-to-H_<II> phase transitions were produced by dehydration due to treatment with hypertonic solutions (both VS and 13S), the membrane vesiculations were produced by biochemical toxicity due to high concentration of EG in the VS.
1991-10-20