P-12 ブラジル産伝承生薬Stryphnodendron fissuratum,Ateleia glaziovianaに含有される化学成分と細胞毒性活性(ポスター発表の部)

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In a continuation of our search for the new bioactive components from Brazilian medicinal herbs, we have now investigated the fruits of Stryphnodendron fissuratum and the leaves of Ateleia glazioviana. Eleven new triterpene saponins whose aglycons are based on the oleanane skeleton (1-6) or the lupane skeleton (7-11) were isolated from the fruits of S. fissuratum, whereas a new isoflavone (12), named glaziovianin A, together with four known isoflavones (13-16) were obtained from the leaves of A. glazioviana. Their structures were determined on the basis of extensive spectroscopic studies, including the ^1H-^1H COSY, selective-TOCSY, HSQC-TOCSY, HMQC, and HMBC spectral data, and by hydrolytic cleavage followed by chromatographic analysis. The isolated compounds were evaluated for their cytotoxic activity against HL-60 human promyelocytic leukemia cells. The isoflavones from A. glazioviana (12-16) showed potent cytotoxic activity (IC_<50> 0.11-7.8μg/mL), among which glaziovianin A (12) was the most effective against HL-60 cells with an IC_<50> value of 0.11μg/mL. When glaziovianin A was subjected to the Jpn. Fdn. for Cancer Res. 39 cell line panel assay, it was cytotoxic to the panel of cancer cells at very low concentrations (-6.18 for the mean values of log GI_<50> levels) with high differential cellular sensitivity; some cell lines such as breast cancer BSY-1 (-7.40), prostate cancer PC-3 (-7.02), lung cancer DMS273 (-6.89), and ovarian cancer OVCAR-3 (-6.87) were especially sensitive to it. Glaziovianin A was suggested to inhibit tublin polymerization by analysis of the differential cellular sensitivity using COMPARE program.
天然有機化合物討論会の論文
2006-09-15

P-12 ブラジル産伝承生薬Stryphnodendron fissuratum,Ateleia glaziovianaに含有される化学成分と細胞毒性活性(ポスター発表の部)

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